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dnase i (Zymo Research)

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Zymo Research dnase i
Dnase I, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/digestion+buffer/DNase+I+Set+w%2F+DNA+Digestion+Buffer/pm42049898-284-15-17
Average 95 stars, based on 486 article reviews

dnase i - by Bioz Stars, 2026-09

95/100 stars

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Immunoprecipitation:

Article Title: A genome language model for mapping DNA replication origins
Article Snippet: Adapter ligated DNA was then denatured and incubated with anti-BrdU antibody (12.5 μg/mL; BD cat. no. 555627), followed by immuno-precipitation with Rabbit anti-mouse IgG (20μg; Sigma-Aldrich, cat. no. M7023). .. Immunoprecipitated DNA was resuspended in digestion buffer with freshly added proteinase K. DNA was purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4013). .. Final libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina and sequenced on an Illumina NextSeq2000 (50bp, single end).

Purification:

Concentration Assay:

Article Title: Spatially resolved gene expression analysis illuminates location-specific functions in the reef-building coral Pocillopora acuta
Article Snippet: .. Caps contained 40 μL of a freshly prepared digestion buffer (95 μL Zymo 2X Digestion Buffer (Zymo Research D3050-1-5), 95 μL RNAse-free water, and 10 μL Proteinase K (20 mg/mL), for a final Proteinase K concentration of 1 mg/mL (Zymo Research D3001-2-5)). ..

Incubation:

Article Title: Redescriptions have consequences: biogeographic and conservation insights from the redescription of a widespread freshwater amphipod (Amphipoda, Gammaridae)
Article Snippet: .. This process began with digestion in a mixture of 400 μL of molecular-grade water, 360 μL of 2× digestion buffer (Zymo Research, Irvine, CA, USA), and 40 μL of Proteinase K; samples were incubated in this mixture at 37 °C for 2 h and then at room temperature overnight. .. Once sufficiently digested, specimens were transferred to a solution of Lignin Pink for 2–3 h to stain; after sufficient staining, specimens were transferred to glycerin for dissection using a Nikon SMZ-800 stereomicroscope.

Article Title: Gateways to the underworld: molecular phylogenetic analyses identify patterns of groundwater fauna and two new species of hypogean Hyalella Smith, 1874 (Amphipoda, Hyalellidae) from the northern Mojave Desert
Article Snippet: .. These individuals were digested in a mixture of 400 μL of Molecular Grade Water, 360 μL of 2× Digestion Buffer (Zymo Research, Irvine, CA, USA), and 40 μL of Proteinase K; samples were incubated in this mixture at 37 °C for 2 hours and then at room temperature overnight. .. Once sufficiently digested, specimens were transferred to a solution of Lignin Pink for 2–3 hours; after specimens were sufficiently stained, they were transferred to glycerin for dissection using a Nikon SMZ-8000 stereomicroscope.